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KMID : 0385920070180020134
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Journal of the Korean Society of Emergency Medicine
2007 Volume.18 No. 2 p.134 ~ p.142
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Effects of α-Lipoic Acid on Apoptotic Cell Death in Rat Hippocampus Following Transient Forebrain Ischemia-reperfusion Injury
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Ahn Hun-Cheol
Song Jin-Ho
Kim Kwang-Seok
Yoo Su-Jin
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Abstract
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Purpose: The purpose of this study was to evaluate effect of α-lipoic acid on apoptotic cell death in rat hippocampal neuron following transient forebrain ischemia-reperfusion (I/R) injury.
Methods: The four-vessel occlusion method was used to induce transient I/R injury in the forebrain of Sprague-
Dawley rats. In the treatment group, α-Lipoic acid (LA) was administered subcutaneously at 50 mg/kg/day for 7 days before induction of I/R injury.
Results: Pretreatment with LA significantly reduced the number of TUNEL-positive neurons in the pyramidal cells
layer of the hipocampal CA1 region 5 days after the ischemia, suggesting a marked reduction of apoptotic cell death. Pretreatment with LA also resulted in marked suppression at the transcript level of mRNA for caspase-3 at 24 hours, and decreased concentration of the active form of caspase-3 protein in the hippocampus at 1, 3, and 5 days after I/R injury. Furthermore, as indicated by western blot analysis, the concentration of apoptosis-inducing factor (AIF) in the hippocampus was reduced at 1 and 3 days after a transient I/R injury by pretreatment with LA.
Conclusion: The results of this study suggest that LA has the potential to prevent neuronal cell death in the hippocampus by inhibiting intracellular signaling pathways responsible for apoptosis following transient I/R injury.
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KEYWORD
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Forebrain ischemia-reperfusion injury, Apoptosis, α-Lipoic acid, Hippocampus
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